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The Journal of Allergy and Clinical Immunology, Volume 132, Issue 4, Pages 777-787, October 2013, Authors:Donald W. MacGlashan, MD, PhD
Via Alfredo Corell
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Rescooped by Gilbert C FAURE from Immunology Diagnosis |
Scoop.it!
Via Alfredo Corell
Gilbert C FAURE's insight:
of course, we lost cytologist's competencies... to look at cells.
We have to rely on secretions assays or cytometric analysis which work pretty well in our experience. Unfortunately, clinical data to correlate biological are not easy to collect and the topic is not very fashionable, somehow outdated.
But, if JACI is devoting a review on the topic, the tests might come back on stage?
Both the treatment of patients with allergic diseases and the study of allergic disease mechanisms depend on a wide variety of assays that in various ways assess the presence and function of IgE antibody. The study of allergic diseases could benefit from the study of its 2 principle cellular participants, mast cells and basophils, but the basophil is more accessible than mast cells for ex vivo studies. Its functionality is tested by using 2 predominant methodologies: the secretion of mediators of allergic inflammation and the expression of proteins on the plasma membrane after stimulation. Each approach has benefits. There are also many operational details to consider regardless of which general approach is taken, and proper interpretation of the methods requires a good understanding of the reagents used and the receptors expressed on basophils and a detailed understanding of the factors regulating aggregation of cell-surface IgE.