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Genetic Engineering Publications - GEG Tech top picks

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Both TALENs and CRISPR/Cas9 directly target the HBB IVS2–654 (C > T) mutation in β-thalassemia-derived iPSCs | Genetic Engineering Publications - GEG Tech top picks | Scoop.it

From www.nature.com - July 16, 2015 10:26 AM

BigField GEG Tech's insight:

The authors designed TALENs and CRISPR/Cas9 to directly target the intron2 mutation site IVS2-654 in the globin gene. They observed that TALENs mediated a higher homologous gene targeting efficiency compared to CRISPR/Cas9 when combined with the piggyBac transposon donor and more obvious off-target events were observed for CRISPR/Cas9 compared to TALENs.

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Both TALENs and CRISPR/Cas9 directly target the HBB IVS2–654 (C > T) mutation in β-thalassemia-derived iPSCs