Genetic Engineering Publications - GEG Tech top picks

The authors  inactivated all three catalytically active DNA methyltransferases (DNMTs) in human embryonic stem cells (ESCs) using CRISPR/Cas9 genome editing. Surprisingly, in contrast to findings in mouse, deletion of DNMT1 resulted in rapid cell death in human ESCs. To overcome this immediate lethality, they generated a doxycycline-responsive tTA-DNMT1 rescue line and readily obtained homozygous DNMT1-mutant lines. However, doxycycline-mediated repression of exogenous DNMT1 initiates rapid, global loss of DNA methylation, followed by extensive cell death.

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