The CRISPR system has been eagerly adopted by many researchers for its ease of use: to edit a genome, all one needs are a Cas9 protein and a single guide RNA (sgRNA) with ~20-nucleotide complementarity to the DNA target site. But this simplicity does not preclude optimization, as Keith Joung and his team from Massachusetts General Hospital now demonstrate by engineering Cas9 proteins with altered targeting ranges.
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The CRISPR system has been eagerly adopted by many researchers for its ease of use: to edit a genome, all one needs are a Cas9 protein and a single guide RNA (sgRNA) with ~20-nucleotide complementarity to the DNA target site. But this simplicity does not preclude optimization, as Keith Joung and his team from Massachusetts General Hospital now demonstrate by engineering Cas9 proteins with altered targeting ranges.
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