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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In this study, the scientists report the use of CRISPR/Cas9 technology to target a specific genomic region in human pluripotent stem cells. They show that CRISPR/Cas9 can be used to disrupt a gene by introducing frameshift mutations to gene coding region; to knock in specific sequences, to targeted genomic locus via homology directed repair and to induce large genomic deletion through dual-guide multiplex. 
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BigField GEG Tech's insight:
Xenotransplantation has been proposed as a solution to the shortage of suitable human donors for transplantation and pigs are currently favoured as donor animals. However, xenotransplantation may be associated with the transmission of zoonotic microorganisms.The zinc finger nuclease (ZFN) technology was used to reduce the risk of horizontal PERV transmission and to knock out as many as possible proviruses.
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BigField GEG Tech's insight:
Single-guide RNA (sgRNA) is one of the two key components of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome-editing system. The current commonly used sgRNA structure has a shortened duplex compared with the native bacterial CRISPR RNA (crRNA)–transactivating crRNA (tracrRNA) duplex and contains a continuous sequence of thymines, which is the pause signal for RNA polymerase III and thus could potentially reduce transcription efficiency
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BigField GEG Tech's insight:
In this work, the authors used the CRISPR/Cas9 system to study the implication of Fbxl3 in circadian rhythms. They show with this tool an efficiency of 70-100% and a role of Fbxl3 in the stability of CRY1.
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In a paper published in Science, Silas et al. now report that a subset of CRISPR systems has the ability to acquire spacers not only from DNA but from RNA as well. The team concludes that the acquisition of RNA spacers by type III CRISPR systems may enable the targeting of parasitic RNA species, such as RNA phages, and may contribute to immune responses towards highly transcribed regions of DNA phages and plasmids via an interference system targeting DNA, RNA or both. In addition, the authors highlight the possibility that RNA spacer acquisition could also occur in species that have seperately encoded RT and Cas1 proteins.