Genetic Engineering Publications - GEG Tech top picks

Researchers at Cardiff University have discovered a new type of killer T-cell that offers hope of a “one-size-fits-all” cancer therapy.

The study was published in Nature Immunology.

https://www.nature.com/articles/s41590-019-0578-8

Here the authors show a proof-of-concept for reprogramming MHC-specificity by performing CRISPR-Cas9-assisted cassette exchange. Using murine antigen presenting cell lines (RAW264.7 macrophages), they demonstrate that the generation of Cas9-induced double-stranded breaks flanking the native MHC-I H2-Kd locus led to exchange of an orthogonal H2-Kb allele. These findings highlight a potential new approach for the correcting of MHC mismatches in cellular transplantation.