Genetic Engineering Publications - GEG Tech top picks

In this study, the authors show that regular FLAG-Cas9 can localize to mitochondria to edit mitochondrial DNA with sgRNAs targeting specific loci of the mitochondrial genome such as Cox1 and Cox3 .Furthermore, to overcome nonspecific distribution of FLAG-Cas9, they also created a mitochondria-targeted Cas9 (mitoCas9) which  could be applied to edit mtDNA together with gRNA expression vectors without affecting genomic DNA.

www.geg-tech.com