After a high-throughput screening, the researchers have identified phorbol 12-myristate 13-acetate (PMA) as a candidate to enhance lentiviral transduction. Indeed, , the percentage of GFP-positive CD34+ cells was increased from 7% in the absence of PMA to greater than 22% in the presence of 1 nM PMA. PMA did not affect colony formation of CD34+ cells or the expression of the hematopoietic markers CD34 and CD45.
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After a high-throughput screening, the researchers have identified phorbol 12-myristate 13-acetate (PMA) as a candidate to enhance lentiviral transduction. Indeed, , the percentage of GFP-positive CD34+ cells was increased from 7% in the absence of PMA to greater than 22% in the presence of 1 nM PMA. PMA did not affect colony formation of CD34+ cells or the expression of the hematopoietic markers CD34 and CD45.
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