Here scientists employ the versatile CRISPR/Cas system to engineer cell lines in which chromosomal translocations are either generated de novo (CD74-ROS1) or existing translocations are reverted back to the original configuration (BCR-ABL1). To this end, they co-apply two guide RNAs to artificially generate two breakpoints and screen for spontaneous fusion events by PCR.
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Here scientists employ the versatile CRISPR/Cas system to engineer cell lines in which chromosomal translocations are either generated de novo (CD74-ROS1) or existing translocations are reverted back to the original configuration (BCR-ABL1). To this end, they co-apply two guide RNAs to artificially generate two breakpoints and screen for spontaneous fusion events by PCR.