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Genetic Engineering Publications - GEG Tech top picks
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In this work the scientists engineered CRISPR/Cas9 system to fuse nuclease deficient Cas9 with the VP64 transactivation domain. They used this tool to target 23 sites within the LTR promoter of HIV-1 and identified a “hotspot” for activation within the viral enhancer sequence. They detected consistent and effective activation of latent virus mediated by activator sgRNAs, whereas latency reversal agents produced variable activation responses. This new generation of tool could represent a promising approach to a “functional cure” of HIV/AIDS. 
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In this work the scientists employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells.