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Multiple strategies are developed to enable multispectral and combinatorial mosaic
gene-function analysis in mice, allowing comparison of molecular phenotypes on a cell-by-cell
basis.
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Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
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Here, the scientists describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.