In this study, the scientists have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. They use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
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In this study, the scientists have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. They use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
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