This protocol describes how to grow untransformed human colonic organoids and deliver genes of interest into the organoids via the piggyBac transposon or gene editing using the CRISPR-Cas9 system.
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This protocol describes how to grow untransformed human colonic organoids and deliver genes of interest into the organoids via the piggyBac transposon or gene editing using the CRISPR-Cas9 system.
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