The authors have demonstrated that Cas9 can be split into two distinct fragments, which form a functional full-length Cas9 nuclease when brought back together by chemical induction. For example, split-Cas9 systems may enable genetic strategies for restricting Cas9 activity to intersections of cell populations by putting each fragment under a different tissue-specific promoter. Additionally, different chemically inducible dimerization domains, such as abscisic acid or gibberellin-sensing domains, may also be employed to generate an array of inducible Cas9 molecules, fused to different modulatory domains, to construct synthetic transcriptional networks.
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The authors have demonstrated that Cas9 can be split into two distinct fragments, which form a functional full-length Cas9 nuclease when brought back together by chemical induction. For example, split-Cas9 systems may enable genetic strategies for restricting Cas9 activity to intersections of cell populations by putting each fragment under a different tissue-specific promoter. Additionally, different chemically inducible dimerization domains, such as abscisic acid or gibberellin-sensing domains, may also be employed to generate an array of inducible Cas9 molecules, fused to different modulatory domains, to construct synthetic transcriptional networks.
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