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Scoop.it!
Light-sensitive regulators of protein kinases could offer valuable insights into intracellular signalling. 
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Scoop.it!
BigField GEG Tech's insight:
The authors identify rules for specific targeting of transcriptional repressors (CRISPRi), and activators (CRISPRa) to endogenous genes via endonuclease-deficient Cas9. Using these rules, they construct genome-scale CRISPRi and CRISPRa libraries. Growth-based screens identify essential genes, tumor suppressors, and regulators of differentiation. These results establish CRISPRi and CRISPRa as powerful tools that provide information for mapping complex pathways.
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Researchers at the Turku Centre for Biotechnology have invented new tools to decode and control signalling circuits in living cells with flashes of light. In principle, any cellular circuit can now be targeted with their method. Using this approach, they discovered that major biological signalling circuits can be made to resonate when driven at their resonant frequency