This study shows simple and efficient methods to manipulate PRV. The scientists established that 100 % viral genes can be disrupt by simple co-transfection of the purified PRV genomes with the CRISPR-associated protein 9 into cells. Furthermore, CRISPR/Cas9-mediated knock-in of >4-kb-long DNA cassettes into the PRV genome at a positive rate of 50 %.
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This study shows simple and efficient methods to manipulate PRV. The scientists established that 100 % viral genes can be disrupt by simple co-transfection of the purified PRV genomes with the CRISPR-associated protein 9 into cells. Furthermore, CRISPR/Cas9-mediated knock-in of >4-kb-long DNA cassettes into the PRV genome at a positive rate of 50 %.
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