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Scoop.it!
Efficient generation of plants carrying mutations in multiple genes remains a challenge. 
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Scoop.it!
CRISPR-Cas9 is used for both enrichment and dropout screens of functional enhancers in human cells.
BigField GEG Tech's insight:
Here the authors present two distinct CRISPR-Cas9 genetic screens to identify and characterize functional enhancers in their native context. They show that a genomic CRISPR-Cas9 tiling screen can precisely map functional domains within enhancer elements. Their approach expands the utility of CRISPR-Cas9 to elucidate the functions of the noncoding genome
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BigField GEG Tech's insight:
Resources for genome-scale loss-of-function screens in mammalian cells have progressed rapidly, particularly with RNA interference (RNAi)-based libraries and, more recently, with CRISPR–Cas9-based libraries. A new parallel screening study leverages the complementary strengths of each system to dissect antiviral drug mechanisms and to identify potential combination therapy strategies.
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BigField GEG Tech's insight:
In this review, the author discuss the basic structure of screens using sleeping beauty to identify numerous cancer drivers in a wide variety of tumor types.
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Here, the scientists describe MISSA 2.0 (multiple-round in vivo site-specific assembly 2.0), an extensively updated toolkit for assembly of two or more CRISPR/Cas systems. They developed a novel suicide donor vector system based on plasmid RK2, which has much higher cloning capacity than the original, plasmid R6K-based system.