Here, the authors find a correlation between free energy potential and the capacity of single-stranded oligonucleotides to inhibit specific DNA cleavage activity, thereby indirectly reducing gene editing activity. These data provide novel information that might be taken into consideration in the design and usage of CRISPR/Cas9 systems with Single-stranded DNA oligonucleotides for gene editing.
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Here, the authors find a correlation between free energy potential and the capacity of single-stranded oligonucleotides to inhibit specific DNA cleavage activity, thereby indirectly reducing gene editing activity. These data provide novel information that might be taken into consideration in the design and usage of CRISPR/Cas9 systems with Single-stranded DNA oligonucleotides for gene editing.
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