Genetic Engineering Publications - GEG Tech top picks

The authors used CRISPR/Cas9 to correct β-Thal iPSCs, gene-corrected cells exhibit normal karyotypes and fully pluripotency as hES, showed no off-targeting effects. Their results shown that the gene-corrected β-Thal iPS cell lines restored HBB expression and reduced reactive oxygen species (ROS) production.

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Obtention of human cartilage from stem cells. Nice!

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The authors used CRISPR-Cas9 and TALEN to edit the genome of human pluripotent stem cell clones and they performed whole-genome sequencing at high coverage in order to assess the degree of mutagenesis across the entire genome. In both types of clones, they found that off-target mutations attributable to the nucleases were very rare. From this analysis, they suggest that, although some cell types may be at risk for off-target mutations, the incidence of such effects in human pluripotent stem cells may be sufficiently low and thus not a significant concern for disease modeling and other applications.

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