Genetic Engineering Publications - GEG Tech top picks

To overcome the low CRISPR-Cas9 editing efficiency at the TERT locus, the scientists develop a two-step “pop-in/pop-out” strategy to enrich cells that underwent homologous recombination (HR). This method provides a useful tool for studying telomerase biology, and suggests a general approach to edit loci with low targeting efficiency and to purify and visualize low abundance proteins.

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As human Rap1, like its mouse and unicellular orthologs, affects gene expression, the authors propose that the conservation of Rap1 reflects its role in transcriptional regulation rather than a function at telomeres.

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