The scientists report a CRISPR/Cas9-based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the control of endogenous promoter, using the TGFβ-responsive gene PAI-1. Using chemical and genetic regulators of TGFβ signalling, they show that it mimics the transcriptional regulation of endogenous PAI-1 expression.
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The scientists report a CRISPR/Cas9-based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the control of endogenous promoter, using the TGFβ-responsive gene PAI-1. Using chemical and genetic regulators of TGFβ signalling, they show that it mimics the transcriptional regulation of endogenous PAI-1 expression.
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