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Genetic Engineering Publications - GEG Tech top picks
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Scoop.it!
This introduction video shows how the choice of every vector element can modify the lentiviral vector's activity.
BigField GEG Tech's insight:
Video which explains the influence of promoter, integrase or envelope on the activity of lentiviral vectors. The goal is to design the best vector according to the experimental needs. 
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The authors describe methods for rapid synthesis of gRNA and for delivery of Cas9 protein/gRNA ribonucleoprotein complexes (Cas9 RNPs) into a variety of mammalian cells through liposome-mediated transfection or electroporation. Using these methods, they report nuclease-mediated indel rates of up to 94% in Jurkat T cells and 87% in induced pluripotent stem cells (iPSC) for a single target.
www.geg-tech.com/Vectors