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Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutation
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Whether CRISPR system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here the scientists demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, they demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, their results also reveal limitations of this correction procedure and highlight the need for further research.