Research and publish the best content.
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Already have an account: Login
Genetic Engineering Publications - GEG Tech top picks
36.6K views |
+0 today
 Your new post is loading...
Your new post is loading...
|
Scoop.it!
BigField GEG Tech's insight:
The authors inactivated all three catalytically active DNA methyltransferases (DNMTs) in human embryonic stem cells (ESCs) using CRISPR/Cas9 genome editing. Surprisingly, in contrast to findings in mouse, deletion of DNMT1 resulted in rapid cell death in human ESCs. To overcome this immediate lethality, they generated a doxycycline-responsive tTA-DNMT1 rescue line and readily obtained homozygous DNMT1-mutant lines. However, doxycycline-mediated repression of exogenous DNMT1 initiates rapid, global loss of DNA methylation, followed by extensive cell death. 
|
This study demonstrates a knockin strategy without drug selection for both active and silent genes in human embryonic stem cells (hESCs) thanks to the iCRISPR platform. This selection-free knockin strategy is expected to greatly facilitate the use of hESCs for developmental studies, disease modeling, and cell-replacement therapy.
www.geg-tech.com/Vectors