Genetic Engineering Publications - GEG Tech top picks

Here, the authors characterize the effect of different culture temperatures on CRISPR-Cas9 mediated genome editing and find that the genome editing efficiency of CRISPR-Cas9 is significantly hampered by hypothermia treatment, unlike ZFN and TALEN. In addition, hyperthermia culture condition enhances genome editing by CRISPR-Cas9 in some cell lines, due to the higher enzyme activity and sgRNA expression level at higher temperature. This study has implications on CRISPR-Cas9 applications in a broad spectrum of species, many of which do not live at 37 °C.