Genetic Engineering Publications - GEG Tech top picks

Recent advances in gene editing with engineered nucleases have transformed our ability to manipulate the genome from diverse organisms for applications ranging from biomedical research to disease treatment. A major complication of use is the off-target of these engineered nucleases which is followed by DNA repair using normal cellular DNA repair mechanisms and which causes gene mutation or gross chromosome rearrangement. Here the authors reviewed developments will enable gene editing tools to be applied more broadly and safely in basic research and disease treatment.

In this study, the scientists identified and characterized new non-conventional RVDs (ncRVDs) that possess novel intrinsic targeting specificity features. By implementing such ncRVDs, they demonstrated in living cells the possibility to efficiently promote TALEN-mediated processing of a target in the HBB locus and alleviate undesired off-site cleavage.

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A major complication with genome editing toolss is the binding of the nuclease to unintended genomic sites that share sequence homology with the on-target site. Here the authors reviewed the significant progress has been made recently to boost the nuclease targeting specificity by protein engineering to modify the structure of the nuclease and alter the interaction with its genomic target.