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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In the recent decades, human genome engineering has been one of the major interesting research subjects, essentially because it raises new possibilities for personalized medicine and biotechnologies. In this review, the authors present the recent advances regarding human genome edition tools, discuss the numerous implications they have in research and medicine, and mention the limits and concerns about such technologies 
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BigField GEG Tech's insight:
Here, the authors review the applications of programmable nucleases in biology and medicine with a focus on CRISPR system. They discuss about the impact of the unprecedented ability to modify cells offer by CRISPR to the scientists and about the consequence of the off targets.
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BigField GEG Tech's insight:
The authors demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique gRNAs targeting all VACV genes will be valuable for the study of VACV gene functions.
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BigField GEG Tech's insight:
Developing a highly productive cell line is a balancing act. Due weight must be given to various factors, particulary clone selection and media feed. And yet more finesse will be required if bioprocessors are to get the most out of the newer cell lines, many of which are bound to embody the most subtle shifts gene-editing technologies such as CRISPR can allow.
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BigField GEG Tech's insight:
Here, the scientists developed a ligation-independent cloning (LIC) assembly method for efficient and bias-free generation of large sgRNA libraries. They tested their selection constructs in HEK 293T cells and showed a median genome editing activity of 54.6% and their shRNA of this library to generate knockout cells was successful for 19 out of 19 genes tested.
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BigField GEG Tech's insight:
Chinese hamster ovary (CHO) cells are the most widely used production host for therapeutic proteins. With the recent emergence of CHO genome sequences, CHO cell line engineering has taken on a new aspect through targeted genome editing.
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Purpose of review: This review will highlight some of the recent advances in genome engineering with applications for both clinical and basic science investigations of HIV-1.
BigField GEG Tech's insight:
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A major complication with genome editing toolss is the binding of the nuclease to unintended genomic sites that share sequence homology with the on-target site. Here the authors reviewed the significant progress has been made recently to boost the nuclease targeting specificity by protein engineering to modify the structure of the nuclease and alter the interaction with its genomic target.