Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
 Your new post is loading...
Your new post is loading...
Scoop.it!
T-reg or T-regulatory cells have direct roles in both autoimmunity and responses to pathogens. Here we'll show you how to quantify Tregs. 
No comment yet.
Sign up to comment
Scoop.it!
Forkhead box P3 (FoxP3)+ CD4+ T cells, known as regulatory T cells or TREGs, are a class of negative regulatory T cells that function to suppress immune responses, thereby establishing tolerance, preventing autoimmunity, and allowing tumor escapes...
Alfredo Corell's insight:
Again an excellent "State of the art" for identifying regulatory T cells by flow cytometry.
|
Scoop.it!
Genetic profiling to identify specific cell surface markers for a highly suppressive Treg population.
Alfredo Corell's insight:
Tr1 cells uniquely expressed genes for two cell surface markers, CD49b and LAG-3.
|
The standard Treg gating strategy for both mouse and human samples (after first gating out doublets and gating on live cells) includes the antigens CD3, CD4, CD25, FOXP3, and CD127.
When looking solely at antigen expression, Tregs are often defined as CD3+, CD4+, CD25hi, FOXP3+, and CD127lo (shown in the figure below as Option 1). Using these markers, a clear population is often visible from samples such as mouse splenocytes and human PBMC.