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Medical Laboratory Observer Amid indications that vitamin D deficiency is increasing, clinical laboratories should conduct an informed consideration of available test options.
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The results reported for a total vitamin D assay may differ based on the method used. Immunoassay methods report a single total vitamin D result; high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) methods report vitamin D2 and vitamin D3 values separately and the two values are added together to obtain the total vitamin D value. Current scientific literature does not indicate that there is a clinical advantage to the latter approach, as it is the total 25(OH)D value that drives a clinician’s decision to treat.