Abstract Background: Fingolimod inhibits lymphocyte egress from lymphoid tissues, thus altering the composition of the peripheral lymphocyte pool of multiple sclerosis patients.
Objective: The objective of this paper is to evaluate whether fingolimod determines a decrease of newly produced T- and B-lymphocytes in the blood and a reduction in the T-cell receptor repertoire diversity that may affect immune surveillance.
Methods: Blood samples were obtained from multiple sclerosis patients before fingolimod therapy initiation and then after six and 12 months. Newly produced T and B lymphocytes were measured by quantifying T-cell receptor excision circles and K-deleting recombination excision circles by real-time PCR, while recent thymic emigrants, naive CD8+ lymphocytes, immature and naive B cells were determined by immune phenotyping. T-cell receptor repertoire was analyzed by complementarity determining region 3 spectratyping.
Results: Newly produced T and B lymphocytes were significantly reduced in peripheral blood of fingolimod-treated patients. The decrease was particularly evident in the T-cell compartment. T-cell repertoire restrictions, already present before therapy, significantly increased after 12 months of treatment.
Conclusions: These results do not have direct clinical implications but they may be useful for further understanding the mode of action of this immunotherapy for multiple sclerosis patients.
Via
Krishan Maggon
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Cytokine
Volume 73, Issue 2, June 2015, Pages 236–244
Review Article Seeking balance: Potentiation and inhibition of multiple sclerosis autoimmune responses by IL-6 and IL-10Sara J. Irelanda, 1, 3, , Nancy L. Monsona, 2, 3, , , Laurie S. Davisb, , 3, Show moreChoose an option to locate/access this article:Check if you have access through your login credentials or your institutionCheck access Purchase $35.95 doi:10.1016/j.cyto.2015.01.009