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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection platforms currently report qualitative results. However, technology based on RT-PCR allows for calculation of viral load, which is associated with transmission risk and disease severity in other viral illnesses. Viral load in COVID-19 might correlate with infectivity, disease phenotype, morbidity, and mortality. To date, no studies have assessed the association between viral load and mortality in a large patient cohort. To our knowledge, we are the first to report on SARS-CoV-2 viral load at diagnosis as an independent predictor of mortality in a large hospitalised cohort (n=1145).
We prospectively evaluated nasopharyngeal swab samples for SARS-CoV-2 by real-time RT-PCR (Roche cobas 6800; Roche, Basel, Switzerland). Positive samples were assessed by a laboratory-developed quantitative RT-PCR test approved for clinical use and viral loads were calculated with standard curves. Viral loads for symptomatic, hospitalised patients who tested positive for SARS-CoV-2 were measured on samples collected between March 13 and May 4, 2020, that tested positive on both platforms at diagnosis. Only patients with complete survival data (discharged from or died in hospital) were included in our analysis (n=1145). Mean age was 64·6 years (SD 17·5), with 651 (56·9%) male patients, and a self-reported racial distribution of 357 (31·2%) African American patients, 335 (29·3%) white patients, 42 (3·7%) Asian patients, 375 (32·8%) patients of other race, and 36 (3·1%) patients of unknown race. The overall mean log10 viral load was 5·6 copies per mL (SD 3·0), and median log10 viral load was 6·2 copies per mL (IQR 3·0–8·0). Mean log10 viral load significantly differed between patients who were alive (n=807; mean log10 viral load 5·2 copies per mL [SD 3]) versus those who had died (n=338; 6·4 copies per mL [2·7]) by the end of the study period.
A Cox proportional hazards model adjusting for age, sex, asthma, atrial fibrillation, coronary artery disease, chronic kidney disease, chronic obstructive pulmonary disease, diabetes, heart failure, hypertension, stroke, and race yielded a significant independent association between viral load and mortality (hazard ratio 1·07 [95% CI 1·03–1·11], p=0·0014; appendix p 3), with a 7% increase in hazard for each log transformed copy per mL. A univariate survival analysis revealed a significant difference in survival probability between those with high viral load (defined as being greater than the overall mean log10 viral load of 5·6 copies per mL) and those with low viral load (p=0·0003; appendix p 4), with a mean follow-up of 13 days (SD 11) and a maximum follow-up of 67 days.
Published at the Lancet (August 6, 2020):
https://doi.org/10.1016/S2213-2600(20)30354-4
